Production of monoclonal antibodies against bovine parvovirus

Monoclonal antibodies [MAbs] against bovine parvovirus [BPV] were successfully produced after fusing the spleen cells of an immunized mouse with myelorna cells. A total of six hybridomas were found to produce MAbs against BPV with different reactivities using heamagglutination inhibition [HI] test. These clones are: 3B[10], 3C[5], 3D[6], 2H[6] and 1E[6]. The first three clones produced MAbs of IgG class, but the last three clones produced those of the IgM class and all of them contained K light chain